Difference between revisions of "Part:BBa K3600014"
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<partinfo>BBa_K3600014 short</partinfo> | <partinfo>BBa_K3600014 short</partinfo> | ||
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This cassette is the product of a two part (BBa_K3600007, BBa_K3600012) Golden Gate BsmBI assembly. | This cassette is the product of a two part (BBa_K3600007, BBa_K3600012) Golden Gate BsmBI assembly. | ||
Latest revision as of 02:19, 28 October 2020
Lys2-TRX2pr-mScarleti cassette
This cassette is the product of a two part (BBa_K3600007, BBa_K3600012) Golden Gate BsmBI assembly.
Usage and Biology
This cassette will be integrated into the yeast genome to form a reporter strain that produces a fluorescent signal when the yeast cell is under environmental stress.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1184
Illegal EcoRI site found at 1914
Illegal XbaI site found at 1193
Illegal XbaI site found at 1618
Illegal XbaI site found at 2091
Illegal SpeI site found at 3059
Illegal PstI site found at 3068 - 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1184
Illegal EcoRI site found at 1914
Illegal XbaI site found at 1193
Illegal XbaI site found at 1618
Illegal XbaI site found at 2091
Illegal SpeI site found at 3059
Illegal PstI site found at 3068 - 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]