Difference between revisions of "Part:BBa K3600012"

Latest revision as of 02:07, 28 October 2020

Lys2-GFP dropout multi-gene backbone

This part is composed of two Lys2 homology sequences (3' and 5') that allow integration of cassettes into the yeast genome. It was synthesized by the EPFL LBNC lab.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1064
Illegal EcoRI site found at 1309
Illegal XbaI site found at 1009
Illegal XbaI site found at 1318
Illegal SpeI site found at 2687
Illegal PstI site found at 2696
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1064
Illegal EcoRI site found at 1309
Illegal SpeI site found at 2687
Illegal PstI site found at 2696
Illegal NotI site found at 793
Illegal NotI site found at 3251
21
INCOMPATIBLE WITH RFC[21]
Illegal suffix found in sequence at 2723
Illegal EcoRI site found at 1064
Illegal EcoRI site found at 1309
Illegal BglII site found at 2711
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1064
Illegal EcoRI site found at 1309
Illegal XbaI site found at 1009
Illegal XbaI site found at 1318
Illegal SpeI site found at 2687
Illegal PstI site found at 2696
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1064
Illegal EcoRI site found at 1309
Illegal XbaI site found at 1009
Illegal XbaI site found at 1318
Illegal SpeI site found at 2687
Illegal PstI site found at 2696
Illegal NgoMIV site found at 2612
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2501
Illegal BsaI.rc site found at 1483
Illegal SapI.rc site found at 826
Illegal SapI.rc site found at 1667

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3600012 short</partinfo>
-This part is composed of two Lys2 homology sequences (3' and 5') that allow integration of cassettes into the yeast genome.
+This part is composed of two Lys2 homology sequences (3' and 5') that allow integration of cassettes into the yeast genome. It was synthesized by the EPFL LBNC lab.
 <!-- Add more about the biology of this part here