Difference between revisions of "Part:BBa K3425107"

 
 
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<partinfo>BBa_K3425107 short</partinfo>
 
<partinfo>BBa_K3425107 short</partinfo>
  
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BBa_K3425101 followed downstream by mRFP1 (BBa_E1010)in pSB1C3.
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BBa K3425107 is a pCadBA testing construct containing the pCadBA operator and promoter BBa_K3425101 followed downstream by the RBS BBa_B0034 and mRFP1 (BBa_E1010) expressed in pSB3C3
  
 
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Latest revision as of 01:55, 28 October 2020


pCadBA testing construct (pSB1C3)

BBa K3425107 is a pCadBA testing construct containing the pCadBA operator and promoter BBa_K3425101 followed downstream by the RBS BBa_B0034 and mRFP1 (BBa_E1010) expressed in pSB3C3

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 2787
    Illegal AgeI site found at 2899
  • 1000
    COMPATIBLE WITH RFC[1000]