Difference between revisions of "Part:BBa K3425106"

 
 
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<partinfo>BBa_K3425106 short</partinfo>
 
<partinfo>BBa_K3425106 short</partinfo>
  
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BBa_K3425101 followed downstream by mRFP1 (BBa_E1010)in pSB3K3
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BBa K3425106 is a pCadBA testing construct containing the pCadBA operator and promoter BBa_K3425101 followed downstream by the RBS BBa_B0034 and mRFP1 (BBa_E1010) expressed in pSB3K3.
  
 
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Latest revision as of 01:54, 28 October 2020


pCadBA testing construct (pSB3K3)

BBa K3425106 is a pCadBA testing construct containing the pCadBA operator and promoter BBa_K3425101 followed downstream by the RBS BBa_B0034 and mRFP1 (BBa_E1010) expressed in pSB3K3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2729
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2729
    Illegal NheI site found at 1384
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2729
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2729
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2729
    Illegal XbaI site found at 2744
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 1470
    Illegal AgeI site found at 1793
    Illegal AgeI site found at 3467
    Illegal AgeI site found at 3579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2498