Difference between revisions of "Part:BBa K165020"
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This is a synthetic transcriptional activator for use in yeast.  It contains the VP16 activation domain, the LexA DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator.  It binds to the Lex operator, activating transcription downstream.  
 
This is a synthetic transcriptional activator for use in yeast.  It contains the VP16 activation domain, the LexA DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator.  It binds to the Lex operator, activating transcription downstream.  
  
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This biobrick was improved upon by the UCopenhagen 2020 team. The improvement consists of fusing the biobrick to a transmembrane domain linked by a flexible link containing a TEV protease cleavage site. The improved biobrick can be found at <bbpart>BBa_K3617007</bbpart>.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K165020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K165020 SequenceAndFeatures</partinfo>
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===Improvement by UCopenhagen2020===
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This biobrick was improved upon by the UCopenhagen 2020 team. The improvement consists of fusing the biobrick to a transmembrane domain linked by a flexible link containing a TEV protease cleavage site as well as adding a ER import signal. This makes the synthetic transcription factor localize to the membrane. If a TEV protease cleaves the biobrick, the synthetic transcription factor is able to relocate to the nucleus and induce expression of a reporter gene under control of a LexAop promoter. The improved biobrick can be found at <bbpart>BBa_K3617007</bbpart>.
  
  

Revision as of 01:53, 28 October 2020


LexA Synthetic Activator (untagged)

This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the LexA DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Lex operator, activating transcription downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Improvement by UCopenhagen2020

This biobrick was improved upon by the UCopenhagen 2020 team. The improvement consists of fusing the biobrick to a transmembrane domain linked by a flexible link containing a TEV protease cleavage site as well as adding a ER import signal. This makes the synthetic transcription factor localize to the membrane. If a TEV protease cleaves the biobrick, the synthetic transcription factor is able to relocate to the nucleus and induce expression of a reporter gene under control of a LexAop promoter. The improved biobrick can be found at BBa_K3617007.