Difference between revisions of "Part:BBa K3629019"
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| − | 1. | + | 1. KEGG PATHWAY: Valine, leucine and isoleucine biosynthesis. (n.d.). Retrieved October 26, 2020, from https://www.genome.jp/kegg-bin/show_pathway?yli00290 |
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Latest revision as of 01:50, 28 October 2020
Yarrowia lipolytica LEU4 gene
2-isopropylmalate (LEU4) coding sequence from Yarrowia lipolytica .
Usage and Biology
2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a leucine binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate leucine synthesis and produce a leucine overproducing strain.
Design
Below is the codon-optimized sequence of Yarrowia lipolytica LEU4 for use in any expression construct. However, we have also made our own expression construct BBa_K3629023, designed to allow for optimal expression of LEU4 in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 728
Illegal BamHI site found at 293
Illegal XhoI site found at 428 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 799
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 544
References
1. KEGG PATHWAY: Valine, leucine and isoleucine biosynthesis. (n.d.). Retrieved October 26, 2020, from https://www.genome.jp/kegg-bin/show_pathway?yli00290