Difference between revisions of "Part:BBa K3425074"

 
 
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<partinfo>BBa_K3425074 short</partinfo>
 
<partinfo>BBa_K3425074 short</partinfo>
  
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HSP 16.3 is a heat shock protein commonly used as biomarker of Mycobacterium tuberculosis.  
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BBa K3425074 is a Constitutive expression construct of a his-tagged HSP 16.3 protein and is constructed from the constitutive promoter BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the his-tag equipped HSP 16.3, BBa_K3425073, as well as the double terminator BBa_B0015.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:24, 28 October 2020


Expression construct of HSP 16.3 with His tag

BBa K3425074 is a Constitutive expression construct of a his-tagged HSP 16.3 protein and is constructed from the constitutive promoter BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the his-tag equipped HSP 16.3, BBa_K3425073, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]