Difference between revisions of "Part:BBa K3589107:Design"

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===Source===
 
===Source===
  
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This part originates from <I>Botrytis aclada</I> (Yang, Q., 2018) and is condon-optimized for Chlamydomonas reinhardtii.
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This part originates from <I>Botrytis aclada</I> (Yang, Q., 2018) and is condon-optimized for <I>Chlamydomonas reinhardti</I>i.
  
 
===References===
 
===References===

Revision as of 01:19, 28 October 2020


Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii, Part 1 (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1055
    Illegal BamHI site found at 1394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of the marine bacteria was removed (?). This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.



Source

This part originates from Botrytis aclada (Yang, Q., 2018) and is condon-optimized for Chlamydomonas reinhardtii.

References