Difference between revisions of "Part:BBa K3657001:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | Pumilio Domains are specific for the different RNA bases, by varying | + | Pumilio Domains are specific for the different RNA bases, by varying the amino acids at position 16 and 20. For the guanine binding Pumby Domain, they are serine and glutamic acid, respectively. For more information on the design, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p> |
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===Source=== | ===Source=== | ||
| − | Drosophila | + | <i>Drosophila melanogaster</i> (Part of the Puf protein) |
===References=== | ===References=== | ||
Latest revision as of 01:10, 28 October 2020
RNA binding Pumilio Homology Domain (Guanin)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Pumilio Domains are specific for the different RNA bases, by varying the amino acids at position 16 and 20. For the guanine binding Pumby Domain, they are serine and glutamic acid, respectively. For more information on the design, visit iGEM Heidelberg 2020
Source
Drosophila melanogaster (Part of the Puf protein)
References
Shuyun Dong and Yang Wang and C. Cassidy-Amstutz and Gang Lu and R. Bigler and Mark R. Jezyk and C. Li and T. Hall and Z. Wang (2011) Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains*.The Journal of Biological Chemistry 286, 26732 - 26742
Katarzyna P Adamala and D. Martin-Alarcon and E. Boyden (2016) Programmable RNA-binding protein composed of repeats of a single modular unit.Proceedings of the National Academy of Sciences 113, E2579 - E2588