Difference between revisions of "Part:BBa K3408001"
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| − | Phytase is a kind of phosphate-solubilizing enzyme, which can hydrolyze phytate to | + | Phytase is a kind of phosphate-solubilizing enzyme, which can hydrolyze phytate to release phosphate. |
Considering the limitation of pH, we chose the phytase which could work efficiently under pH 6.0 to pH 7.0. We have found the <i>phy(ycD)</i> from <i>Bacillus subtilis</i> YCD whose optimum reaction pH is 6.5(Fig.1). Thus, we constructed <i>phy(ycD)</i> gene into our engineered bacteria. | Considering the limitation of pH, we chose the phytase which could work efficiently under pH 6.0 to pH 7.0. We have found the <i>phy(ycD)</i> from <i>Bacillus subtilis</i> YCD whose optimum reaction pH is 6.5(Fig.1). Thus, we constructed <i>phy(ycD)</i> gene into our engineered bacteria. | ||
Latest revision as of 01:08, 28 October 2020
Phy(ycD)(Phytase)
Phytase is a kind of phosphate-solubilizing enzyme, which can hydrolyze phytate to release phosphate.
Considering the limitation of pH, we chose the phytase which could work efficiently under pH 6.0 to pH 7.0. We have found the phy(ycD) from Bacillus subtilis YCD whose optimum reaction pH is 6.5(Fig.1). Thus, we constructed phy(ycD) gene into our engineered bacteria.
Fig.1. Effects of pH on the phytase activity (Wang Xi, et al., Applied and Environmental Biology. 2014)
According to the paper, we could see its optimum reaction pH is 6.5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 359
- 1000COMPATIBLE WITH RFC[1000]