Difference between revisions of "Part:BBa K3606114"

Latest revision as of 00:59, 28 October 2020

P3 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P3 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.

design:

In this part, the expression of tetR is driven by LuxpR; tetR will block ptetR, resulting in the inability to express McbEFG; and P3 as a constitutive promoter will continue to express McbABCD. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 5317
Illegal PstI site found at 2338
Illegal PstI site found at 2371
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 5274
Illegal PstI site found at 2338
Illegal PstI site found at 2371
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2245
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 5317
Illegal PstI site found at 2338
Illegal PstI site found at 2371
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 5317
Illegal PstI site found at 2338
Illegal PstI site found at 2371
Illegal NgoMIV site found at 2000
Illegal AgeI site found at 2172
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P3 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.
+<h2>design:</h2>
+In this part, the expression of tetR is driven by LuxpR; tetR will block ptetR, resulting in the inability to express McbEFG; and P3 as a constitutive promoter will continue to express McbABCD.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===