Difference between revisions of "Part:BBa K165068"
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<partinfo>BBa_K165068 short</partinfo> | <partinfo>BBa_K165068 short</partinfo> | ||
| − | + | Induced by galactose, this activator, tagged with mCherryx2, activates promoters with LexA binding sites. | |
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| + | In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. Under the confocal fluorescence microscope, we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below: | ||
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| + | [[Image:Fluorotest.png|center]] | ||
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Revision as of 06:12, 30 October 2008
pGAL1 + LexA activator on pRS305
Induced by galactose, this activator, tagged with mCherryx2, activates promoters with LexA binding sites.
In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. Under the confocal fluorescence microscope, we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]