Difference between revisions of "Part:BBa K3331024"
 
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===Characterization===
 
===Characterization===
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<div>[[File:T--XJTU-China--pBAD-bs-gfp1.png |700px|thumb|center|]]</div>
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<div>[[File:T--XJTU-China--pBAD-bs-gfp1.png |700px|thumb|center|Figure 1:</b> excite the bacteria in 488nm]]</div>
−
<div>[[File:T--XJTU-China--pBAD-bs-gfp2.png |700px|thumb|center|]]</div>
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<div>[[File:T--XJTU-China--pBAD-bs-gfp2.png |700px|thumb|center|Figure 2:</b>detect the absorbance in 537nm]]

Latest revision as of 00:59, 28 October 2020


BS ara operon&GFP

This part comes from the arabinose operon of the Bacillus subtilis 168 genome and is replaced with GFP in the position of the structural gene araE. It contains the regulatory protein araR and its promoter and terminator as well as the promoter and terminator of araE and the reporter gene sfGFP green fluorescent protein gene.We use this part to measure the efficiency of suicide switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization

Figure 1:</b> excite the bacteria in 488nm
Figure 2:</b>detect the absorbance in 537nm