Difference between revisions of "Part:BBa K3425062"

 
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<partinfo>BBa_K3425062 short</partinfo>
 
<partinfo>BBa_K3425062 short</partinfo>
  
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TBD
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BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
  
 
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Revision as of 00:44, 28 October 2020


β-subunit of Qβ replicase under control of inducible pCAD promoter

BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1496
    Illegal XhoI site found at 1004
    Illegal XhoI site found at 1170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]