Difference between revisions of "Part:BBa K3425061"

 
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<partinfo>BBa_K3425061 short</partinfo>
 
<partinfo>BBa_K3425061 short</partinfo>
  
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TBD,
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BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
  
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tends to mutate in high copy number plasmid
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From observations have can it be stated that this construct tends to mutate in high copy number plasmids
  
 
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Revision as of 00:41, 28 October 2020


Constitutive expression construct of Qβ replicase

BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.

From observations have can it be stated that this construct tends to mutate in high copy number plasmids

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1377
    Illegal XhoI site found at 885
    Illegal XhoI site found at 1051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]