Difference between revisions of "Part:BBa K3657005"
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<partinfo>BBa_K3657005 short</partinfo> | <partinfo>BBa_K3657005 short</partinfo> | ||
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+ | This Part is an example of a RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases. | ||
+ | <p> Adenine:<a href="https://parts.igem.org/Part:BBa_K3657000">BBa_K3657000</a><p> | ||
+ | Guanine:<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a><p> | ||
+ | Uracil:<a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a><p> | ||
+ | Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p> | ||
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+ | </html> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. | ||
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Revision as of 00:39, 28 October 2020
RNA binding Pumby Protein - Motif AUGGUU
This Part is an example of a RNA-binding Pumby Protein, assembled with the Pumby Domains that are specific for the four RNA bases.
Adenine:BBa_K3657000
Guanine:BBa_K3657001
Uracil:BBa_K3657002
Cytosine:BBa_K3657003
Usage and Biology
Pumby Proteins are assembled by combining multiple RNA-base specific RNA binding Domains. They originate from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 184
Illegal XhoI site found at 517
Illegal XhoI site found at 628 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]