Difference between revisions of "Part:BBa K3475003"

Latest revision as of 00:34, 28 October 2020

Prm RBS HrtR Ter

Part of heme biosensor system. HrtO repressor, stop repressing when heme exists

Usage and Biology

FIGURE 2. The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank. From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM.

FIGURE 3. Calculating formula.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 364
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 364
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 364
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 <div style="text-align:center">[[Image:T--Tongji_China--202_2.jpg]]</div>
 <p style="width:600px; margin-left:150px; margin-bottom:60px;
-text-align:justify "><em><strong>FIGURE 2.</strong> '''The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).'''<br/>Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank. </em> </p>
+text-align:justify "><em><strong>FIGURE 2.</strong> '''The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).'''<br/>Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank. From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM.  </em> </p>
 <div style="text-align:center">[[Image:T--Tongji_China--202_t.jpg|650px]]</div>
 <p style="width:600px; margin-left:150px; margin-bottom:60px;
-text-align:justify "><em><strong>FIGURE 3.</strong> '''From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM. </em> </p>
+text-align:justify "><em><strong>FIGURE 3.</strong> '''Calculating formula.</em> </p>