Difference between revisions of "Part:BBa K3606915"

Latest revision as of 00:32, 28 October 2020

P2 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P2 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.

design:

In this part, the expression of tetR is driven by LuxpR; tetR will block ptetR, resulting in the inability to express McbEFG; and P2 as a constitutive promoter will continue to express McbABCD.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 5276
Illegal PstI site found at 2297
Illegal PstI site found at 2330
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 5233
Illegal PstI site found at 2297
Illegal PstI site found at 2330
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 5276
Illegal PstI site found at 2297
Illegal PstI site found at 2330
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 5276
Illegal PstI site found at 2297
Illegal PstI site found at 2330
Illegal NgoMIV site found at 1959
Illegal AgeI site found at 2131
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P2 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.
+<h2>design:</h2>
+In this part, the expression of tetR is driven by LuxpR; tetR will block ptetR, resulting in the inability to express McbEFG; and P2 as a constitutive promoter will continue to express McbABCD.