Difference between revisions of "Part:BBa K3606916:Design"

 
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===Design Notes===
 
===Design Notes===
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tba
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This part completely integrates our quorum-sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P11 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.
  
  

Revision as of 00:27, 28 October 2020


P11 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5306
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5263
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5306
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5306
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
    Illegal NgoMIV site found at 1989
    Illegal AgeI site found at 2161
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part completely integrates our quorum-sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P11 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.


Source

tba

References