Difference between revisions of "Part:BBa K3588020"
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All design details are detailed in the paper 'Programming Controlled Adhesion of E. coli to Target Surfaces, Cells, and Tumors with Synthetic Adhesins'<ref>https://pubs.acs.org/doi/abs/10.1021/sb500252a</ref> by the Department of Microbial Biotechnology in Spain. They detail how the protein was based off of natural E. coli adhesins: an anchor Beta-barrel module that is embedded in the membrane and an adhesion module bearing an immunoglobulin (Ig) like fold. This synthetic adhesin combines the stable Beta-barrel domain derived from intimin found in EHEC and EPEC E. coli strains. The Ig domain was based on VHH library, a series of variable domains of defined specificity against antigens of interest. | All design details are detailed in the paper 'Programming Controlled Adhesion of E. coli to Target Surfaces, Cells, and Tumors with Synthetic Adhesins'<ref>https://pubs.acs.org/doi/abs/10.1021/sb500252a</ref> by the Department of Microbial Biotechnology in Spain. They detail how the protein was based off of natural E. coli adhesins: an anchor Beta-barrel module that is embedded in the membrane and an adhesion module bearing an immunoglobulin (Ig) like fold. This synthetic adhesin combines the stable Beta-barrel domain derived from intimin found in EHEC and EPEC E. coli strains. The Ig domain was based on VHH library, a series of variable domains of defined specificity against antigens of interest. | ||
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Revision as of 00:23, 28 October 2020
Synthetic Adhesin
This part is expressed on the extracellular membrane of E. Coli and from there has a an ability to adhere to an antigenic surface.
function
The part, when expressed correctly, allows for an increased ability for adherence to antigenic surfaces. The antigenic surface can be any common protein provided the synthetic adhesin VHH region is modified to be complimentary. In this case the VHH region has a complimentary binding site of GFP, and so if GFP is fixed to a surface, such as an ELISA plate then any bacteria expressing a complimentary binding region will be better able to adhere to given surface. This can be seen in the image included here but is better seen through our lab work results: https://2020.igem.org/Team:CLS_CLSG_UK/Results
design
All design details are detailed in the paper 'Programming Controlled Adhesion of E. coli to Target Surfaces, Cells, and Tumors with Synthetic Adhesins'[1] by the Department of Microbial Biotechnology in Spain. They detail how the protein was based off of natural E. coli adhesins: an anchor Beta-barrel module that is embedded in the membrane and an adhesion module bearing an immunoglobulin (Ig) like fold. This synthetic adhesin combines the stable Beta-barrel domain derived from intimin found in EHEC and EPEC E. coli strains. The Ig domain was based on VHH library, a series of variable domains of defined specificity against antigens of interest.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 105
Illegal PstI site found at 401
Illegal PstI site found at 649
Illegal PstI site found at 1096 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 105
Illegal PstI site found at 401
Illegal PstI site found at 649
Illegal PstI site found at 1096
Illegal NotI site found at 2389 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 105
Illegal BamHI site found at 1983 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 105
Illegal PstI site found at 401
Illegal PstI site found at 649
Illegal PstI site found at 1096 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 105
Illegal PstI site found at 401
Illegal PstI site found at 649
Illegal PstI site found at 1096
Illegal NgoMIV site found at 2004 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1244
Illegal BsaI site found at 2063