Difference between revisions of "Part:BBa K3410004:Design"

Revision as of 00:11, 28 October 2020

Nanobody Progesterone

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The estradiol nanobody was grafted using an excel sheet and the AHo numbering scheme

Source

Acceptor: cAbBCII-10 [1]

Donor: P15G12C12G11[2,3]

References

[1] C. Vincke, R. Loris, S. Muyldermans, and K. Conrath, Structure of CabBCII-10 nanobody, 2008. [2] C. Monnet et al., Crystal Structure of Fab-Estradiol Complexes, 2002. [3] C. Monnet et al., “Highly specific anti-estradiol antibodies: structural characterisation and binding diversity,” Journal of molecular biology, vol. 315, no. 4, pp. 699–712, 2002, doi: 10.1006/jmbi.2001.5284

@@ Line 8: / Line 8: @@
 ===Design Notes===
 The estradiol nanobody was grafted using an excel sheet and the AHo numbering scheme
 ===Source===
-Acceptor = cAbBCII-10:
+Acceptor: cAbBCII-10 [1]
-C. Vincke, R. Loris, S. Muyldermans, and K. Conrath, Structure of CabBCII-10 nanobody, 2008.
+Donor: P15G12C12G11[2,3]
-Donor = P15G12C12G11:
-C. Monnet et al., Crystal Structure of Fab-Estradiol Complexes, 2002.
-C. Monnet et al., “Highly specific anti-estradiol antibodies: structural characterisation and binding diversity,” Journal of molecular biology, vol. 315, no. 4, pp. 699–712, 2002, doi: 10.1006/jmbi.2001.5284
+===References===
-===References===
+[1] C. Vincke, R. Loris, S. Muyldermans, and K. Conrath, Structure of CabBCII-10 nanobody, 2008.
+[2] C. Monnet et al., Crystal Structure of Fab-Estradiol Complexes, 2002.
+[3] C. Monnet et al., “Highly specific anti-estradiol antibodies: structural characterisation and binding diversity,” Journal of molecular biology, vol. 315, no. 4, pp. 699–712, 2002, doi: 10.1006/jmbi.2001.5284