Difference between revisions of "Part:BBa K3410004:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
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This nanobody is a result of a graft using the cAbBCII-10 nanobody as acceptor and the monoclonal antibody P15G12C12G11 as donor.
+
Acceptor = cAbBCII-10:
 +
C. Vincke, R. Loris, S. Muyldermans, and K. Conrath, Structure of CabBCII-10 nanobody, 2008.
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 +
 
 +
Donor = P15G12C12G11:
 +
C. Monnet et al., Crystal Structure of Fab-Estradiol Complexes, 2002.
 +
C. Monnet et al., “Highly specific anti-estradiol antibodies: structural characterisation and binding diversity,” Journal of molecular biology, vol. 315, no. 4, pp. 699–712, 2002, doi: 10.1006/jmbi.2001.5284
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===References===
 
===References===

Revision as of 00:08, 28 October 2020


Nanobody Progesterone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The estradiol nanobody was grafted using an excel sheet and the AHo numbering scheme


Source

Acceptor = cAbBCII-10: C. Vincke, R. Loris, S. Muyldermans, and K. Conrath, Structure of CabBCII-10 nanobody, 2008.


Donor = P15G12C12G11: C. Monnet et al., Crystal Structure of Fab-Estradiol Complexes, 2002. C. Monnet et al., “Highly specific anti-estradiol antibodies: structural characterisation and binding diversity,” Journal of molecular biology, vol. 315, no. 4, pp. 699–712, 2002, doi: 10.1006/jmbi.2001.5284


References