Difference between revisions of "Part:BBa K3657000:Design"
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===Design Notes===
 
===Design Notes===
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Pumilio Domains are specific for the different RNA bases, by varying in the aminoacid sequence in position 16 and 20. For the Adenine binding Pumby Domain, they are Cysteine and Glutamine, respectively. For more Information on the design, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p>
+
Pumilio Domains are specific for the different RNA bases, by varying in the aminoacid sequence in position 16 and 20. For the Adenine binding Pumby Domain, they are Cysteine and Glutamine, respectively. For more information on the design, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p>
  
 
===Source===
 
===Source===

Revision as of 23:47, 27 October 2020


RNA binding Pumilio Homology Domain (Adenine)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pumilio Domains are specific for the different RNA bases, by varying in the aminoacid sequence in position 16 and 20. For the Adenine binding Pumby Domain, they are Cysteine and Glutamine, respectively. For more information on the design, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p>

Source

Drosophila Melanogaster (Part of the Puf protein)

References

Shuyun Dong and Yang Wang and C. Cassidy-Amstutz and Gang Lu and R. Bigler and Mark R. Jezyk and C. Li and T. Hall and Z. Wang (2011) Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains*.The Journal of Biological Chemistry 286, 26732 - 26742

Katarzyna P Adamala and D. Martin-Alarcon and E. Boyden (2016) Programmable RNA-binding protein composed of repeats of a single modular unit.Proceedings of the National Academy of Sciences 113, E2579 - E2588