Difference between revisions of "Part:BBa K3520019"

 
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<partinfo>BBa_K3520019 short</partinfo>
 
<partinfo>BBa_K3520019 short</partinfo>
  
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&#945;
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[[File:T--Athens--Level_1_design.png|800px|thumb|center|Figure 1: Type IIS for level 1 Golden Gate assembly with SapI.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:44, 27 October 2020


pHimarEm1+TUs of BcsA,B,C,D

Figure 1: Type IIS for level 1 Golden Gate assembly with SapI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 16429
    Illegal suffix found in sequence at 9767
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 16429
    Illegal NheI site found at 10096
    Illegal SpeI site found at 9768
    Illegal PstI site found at 9782
    Illegal NotI site found at 9775
    Illegal NotI site found at 16435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 16429
    Illegal BglII site found at 10418
    Illegal BglII site found at 12957
    Illegal BglII site found at 13616
    Illegal XhoI site found at 10053
    Illegal XhoI site found at 13254
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 16429
    Illegal suffix found in sequence at 9768
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 16429
    Illegal XbaI site found at 16444
    Illegal SpeI site found at 9768
    Illegal PstI site found at 9782
    Illegal NgoMIV site found at 11082
    Illegal AgeI site found at 12649
  • 1000
    COMPATIBLE WITH RFC[1000]