Difference between revisions of "Part:BBa K3699004"
 
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<partinfo>BBa_K3699004 short</partinfo>
 
<partinfo>BBa_K3699004 short</partinfo>
  
 
tRNA:  the pylT gene (tRNA) of <i>Methanosarcina mazei</i>, proK promoter
 
tRNA:  the pylT gene (tRNA) of <i>Methanosarcina mazei</i>, proK promoter
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===Usage and Biology===
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<h3 id="degradation0301">Design</h3>
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<p>
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From virus to human, the same set of genetic code is employed, which contains the start codon, three stop codons (UAA, UAG, UGA), and the remaining 61 codons encoding 20 kinds of amino acids. However, some exceptions have been found, such as pyrrolysyl tRNA synthetase/tRNA (pylrs/tRNA) from <i>Methanosarcina mazei</i>, which can recognize the amber codon UAG and translate it into pyrrolysine. Moreover, in E. coli, yeasts, and mammalian cells, this PylRS/tRNA pair is orthogonal, which means it does not interfere with the translation system of the chassis [1]. The orthogonal translation system has been used in vaccine safety design [2]. Now, we will use it to achieve the anti-escape design of cyanophages.
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</p>
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<h3 id="degradation0301">References</h3>
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<p>[1] Mukai T , Kobayashi T , Hino N , et al. Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases[J]. Biochemical & Biophysical Research Communications, 2008, 371(4):818-822.
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</p>
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<p>[2] Si L , Xu H , Zhou X , et al. Generation of influenza A viruses as live but replication-incompetent virus vaccines.[J]. Science, 2016.
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</p>
  
  
  
 
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===Usage and Biology===
 
  
 
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Latest revision as of 23:09, 27 October 2020

pylT(tRNA)

tRNA: the pylT gene (tRNA) of Methanosarcina mazei, proK promoter

Usage and Biology

Design

From virus to human, the same set of genetic code is employed, which contains the start codon, three stop codons (UAA, UAG, UGA), and the remaining 61 codons encoding 20 kinds of amino acids. However, some exceptions have been found, such as pyrrolysyl tRNA synthetase/tRNA (pylrs/tRNA) from Methanosarcina mazei, which can recognize the amber codon UAG and translate it into pyrrolysine. Moreover, in E. coli, yeasts, and mammalian cells, this PylRS/tRNA pair is orthogonal, which means it does not interfere with the translation system of the chassis [1]. The orthogonal translation system has been used in vaccine safety design [2]. Now, we will use it to achieve the anti-escape design of cyanophages.

References

[1] Mukai T , Kobayashi T , Hino N , et al. Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases[J]. Biochemical & Biophysical Research Communications, 2008, 371(4):818-822.

[2] Si L , Xu H , Zhou X , et al. Generation of influenza A viruses as live but replication-incompetent virus vaccines.[J]. Science, 2016.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]