Difference between revisions of "Part:BBa K3610021:Design"
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Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards. | Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards. | ||
| − | + | The split was made at amino acids 158 and 159 from the full length mCherry. | |
===Source=== | ===Source=== | ||
Revision as of 23:09, 27 October 2020
mCherry C-terminal - codon optimized for S. cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.
The split was made at amino acids 158 and 159 from the full length mCherry.
Source
The sequence was taken from the registry and then adapted for expression in C. reinhardtii