Difference between revisions of "Part:BBa K3629015"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This is the expression construct for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including <i>Yarrowia lipolytica </i> -the chassis for our project. Particularly for <i> Y. lipolytica</i> there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this | + | This is the expression construct for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including <i>Yarrowia lipolytica </i> -the chassis for our project. Particularly for <i> Y. lipolytica</i> there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this selection marker, 50-200µg/mL is added to media depending on the organism. |
The part is a key part of [https://2020.igem.org/Team:Calgary/Part_Collection our collection] and functions as a destination vector for other <i> Y. lipolytica</i> parts when cloned into a plasmid. | The part is a key part of [https://2020.igem.org/Team:Calgary/Part_Collection our collection] and functions as a destination vector for other <i> Y. lipolytica</i> parts when cloned into a plasmid. | ||
Revision as of 23:04, 27 October 2020
Nourseothricin resistance expression construct with gibson homology sequences
Usage and Biology
This is the expression construct for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including Yarrowia lipolytica -the chassis for our project. Particularly for Y. lipolytica there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this selection marker, 50-200µg/mL is added to media depending on the organism.
The part is a key part of our collection and functions as a destination vector for other Y. lipolytica parts when cloned into a plasmid.
Design
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1590
Illegal EcoRI site found at 1449 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1449
Illegal NheI site found at 1584
Illegal SpeI site found at 1591
Illegal PstI site found at 1605
Illegal NotI site found at 7
Illegal NotI site found at 1598 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1449 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1591
Illegal EcoRI site found at 1449 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 1449
Illegal XbaI site found at 16
Illegal SpeI site found at 1591
Illegal PstI site found at 1605 - 1000COMPATIBLE WITH RFC[1000]