Difference between revisions of "Part:BBa K133019"
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The rest of the part consists of: | The rest of the part consists of: | ||
| − | CF213, which is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) | + | CF213, which is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of <i>E. coli</i> flagellin + N-terminal segment of the hypervariable region of <i>H. pylori</i> flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) |
| − | CF215, which is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) | + | CF215, which is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of <i>H. pylori</i> flagellin FlaA from 215 AA + 99 AA of C-terminal portion from <i>E. coli</i> flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) |
| − | We also designed synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt. | + | We also designed synthetic multiepitope, comprising the <i>H. pylori</i> urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt. |
Since the protein was designed to be produced, we additionally added RGD-Hisstop tag. | Since the protein was designed to be produced, we additionally added RGD-Hisstop tag. | ||
Latest revision as of 05:08, 30 October 2008
CMV-CF213-multiHP-CF215-RGD-Histop
We have used CMV promotor, because it is promotor for constitutive expression in mammalian cells.
The rest of the part consists of:
CF213, which is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
CF215, which is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
We also designed synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt.
Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2406
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1963
Illegal BamHI site found at 2474 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 961
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2757