Difference between revisions of "Part:BBa K3699002"

 
 
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<partinfo>BBa_K3699002 short</partinfo>
 
<partinfo>BBa_K3699002 short</partinfo>
  
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To validate the orthogonal translation system, we designed BBa_K3699002. It encodes the putative phage tail sheath protein of Microcystis virus Ma-LMM01. (Protein ID: BAF36182.1)
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To validate the orthogonal translation system, we designed BBa_K3699002. It encodes the putative phage tail sheath protein of <i>Microcystis virus</i> Ma-LMM01. (Protein ID: BAF36182.1)
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We made a base substitution at position 747 (T747G), introducing a general stop codon, UAG, within the gene.In general, the protein expressed by this part is truncated. In the presence of an orthogonal translation system, E. coli would be able to read through the UAG and insert the corresponding unnatural amino acids, restoring the protein to its original length.
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We made a base substitution at position 747 (T747G), introducing a general stop codon, UAG, within the gene.In general, the protein expressed by this part is truncated. In the presence of an orthogonal translation system,<i> E. coli</i> would be able to read through the UAG and insert the corresponding unnatural amino acids, restoring the protein to its original length.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<html>
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        <p>
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            The “tail” protein encodes the putative phage tail sheath protein of <i>Microcystis virus</i> Ma-LMM01.
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            (Protein ID: BAF36182.1)</p>
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        <p>We have made a base substitution at position 747 (T747G), which converted the Tyrosine codon to a stop codon
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            and lead to the early termination of protein translation. In order to facilitate plasmid conctruction, we
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            added BamHI and SacI cutting sites at both ends of the gene.</p>
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        <figure><img src="https://2020.igem.org/wiki/images/5/5c/T--BUCT--eng10.png"/></figure>
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        <figure><img src="https://2020.igem.org/wiki/images/2/24/T--BUCT--eng8.png"/></figure>
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        <p><b>Figure 1. sequence of the tail protein.</b> We made a base substitution at position 747 (T747G), converting TAT
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            to TAG.</p>
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        <p>We speculate that the mutated phage tail sheath protein will not be fully expressed, but a truncated protein.
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        </p>
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</html>
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<!-- Add more about the biology of this part here
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Latest revision as of 22:58, 27 October 2020


the putative phage tail sheath protein of Microcystis virus Ma-LMM01, UAG inserted

To validate the orthogonal translation system, we designed BBa_K3699002. It encodes the putative phage tail sheath protein of Microcystis virus Ma-LMM01. (Protein ID: BAF36182.1) We made a base substitution at position 747 (T747G), introducing a general stop codon, UAG, within the gene.In general, the protein expressed by this part is truncated. In the presence of an orthogonal translation system, E. coli would be able to read through the UAG and insert the corresponding unnatural amino acids, restoring the protein to its original length.

Usage and Biology

The “tail” protein encodes the putative phage tail sheath protein of Microcystis virus Ma-LMM01. (Protein ID: BAF36182.1)

We have made a base substitution at position 747 (T747G), which converted the Tyrosine codon to a stop codon and lead to the early termination of protein translation. In order to facilitate plasmid conctruction, we added BamHI and SacI cutting sites at both ends of the gene.

Figure 1. sequence of the tail protein. We made a base substitution at position 747 (T747G), converting TAT to TAG.

We speculate that the mutated phage tail sheath protein will not be fully expressed, but a truncated protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1185
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 114
    Illegal NgoMIV site found at 1741
  • 1000
    COMPATIBLE WITH RFC[1000]