Difference between revisions of "Part:BBa K3657026"

Revision as of 22:57, 27 October 2020

MS2 Coat Protein + SoxS Trancription Activator
This part is a fusion protein consisting of SoxS activator (BBa_K3657024) and MS2 capsid protein (BBa_K3218006).

Usage and Biology

MS2 capsid protein binds specifically to the MS2 RNA hairpin (BBa_K3113006). SoxS is a powerful activator in E. coli. This construct was used together with a RNA·DNA-DNA triple helix containing an MS2 hairpin (BBa_K3657025). Use of these two parts together allows to attract the SoxS activator to the specific location on a DNA. More information available here: iGEM Heidelberg 2020.

Due to the COVID19 pandemic we were not able to get any empirical evidence about theperformance of this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
 <html>
-MS2 capsid protein binds specifically to the MS2 RNA hairpin. SoxS is a powerful activator in <i>E. coli</i>.
+MS2 capsid protein binds specifically to the MS2 RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3113006">BBa_K3113006</a>). SoxS is a powerful activator in <i>E. coli</i>.
-This construct was used together with a RNA&middotDNA-DNA triple helix containing an MS2 hairpin (<a href="https://parts.igem.org/Part:BBa_K3657025">BBa_K3657025</a>). Use of these two parts together allows to attract the SoxS activator to the specific location on a DNA. More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a>.
+This construct was used together with a RNA&middot;DNA-DNA triple helix containing an MS2 hairpin (<a href="https://parts.igem.org/Part:BBa_K3657025">BBa_K3657025</a>). Use of these two parts together allows to attract the SoxS activator to the specific location on a DNA. More information available here: <a href="https://2020.igem.org/Team:Heidelberg/Triple_Helix">iGEM Heidelberg 2020</a>.
 <p>Due to the COVID19 pandemic we were not able to get any empirical evidence about theperformance of this part.</p>
 </html>