Difference between revisions of "Part:BBa K165096"

Revision as of 05:04, 30 October 2008

Zif268-HIV bs + MET25+ Gli1 repressor (CFPx2 tagged) on pRS304*

This construct is an example of the Tau element of our genetic limiter network. In this case, the pMET25 sets the threshold of limitation, allowing us to tune the threshold by varying the concentration of methionine in the media the cells are grown in. We constructed the Gli1 repressor in this devise with CFPx2 instead of mCherry, allowing us to distinguish it from the other repressors in the network. It is placed in a mutually inhibitory relationship with repressor alpha, in this case the Zif-HIV repressor. When the network is subthreshold, this tau repressor is expressed, and represses R, turning off its repression of the gene of interest. -

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2224
Illegal XhoI site found at 1
Illegal XhoI site found at 41
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3990
Illegal AgeI site found at 4740
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1395
Illegal SapI.rc site found at 1988

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 __NOTOC__
 <partinfo>BBa_K165096 short</partinfo>
+This construct is an example of the Tau element of our genetic limiter network.  In this case, the pMET25 sets the threshold of limitation, allowing us to tune the threshold by varying the concentration of methionine in the media the cells are grown in.  We constructed the Gli1 repressor in this devise with CFPx2 instead of mCherry, allowing us to distinguish it from the other repressors in the network.  It is placed in a mutually inhibitory relationship with repressor alpha, in this case the Zif-HIV repressor.  When the network is subthreshold, this tau repressor is expressed, and represses R, turning off its repression of the gene of interest.
 -