Difference between revisions of "Part:BBa K3380109"
Line 3: Line 3:
 
<partinfo>BBa_K3380109 short</partinfo>
 
<partinfo>BBa_K3380109 short</partinfo>
  
−
Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the native ''Cupriavidus Metallidurans'' CH34 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
+
Zn sensing promoter sequence taken from Millacura, work of Millacura, Valenzuela-Ortega and French 2019
−
 
+
−
Taken from the work of Millacura, Valenzuela-Ortega and French 2019.
+
  
  

Revision as of 22:55, 27 October 2020


C. Metallidurans promoter with Zn transcription factor binding site

Zn sensing promoter sequence taken from Millacura, work of Millacura, Valenzuela-Ortega and French 2019


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]