Difference between revisions of "Part:BBa K3580002"
 
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This is an improvement of BBa_B0015. Instead of GFP([https://parts.igem.org/Part:BBa_E0040 BBa_E0040]), we use our new parts: GFPssrA([https://parts.igem.org/Part:BBa_K3580001 BBa_K3580001]).
 
This is an improvement of BBa_B0015. Instead of GFP([https://parts.igem.org/Part:BBa_E0040 BBa_E0040]), we use our new parts: GFPssrA([https://parts.igem.org/Part:BBa_K3580001 BBa_K3580001]).
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By adding this parts understream of a promoter, it can express GFP with degradation tag.
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By adding this parts understream of a promoter, it can express GFP with a degradation tag.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:52, 27 October 2020


GFPssrA with RBS and double terminator

This is an improvement of BBa_B0015. Instead of GFP(BBa_E0040), we use our new parts: GFPssrA(BBa_K3580001). By adding this parts understream of a promoter, it can express GFP with a degradation tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 656