Difference between revisions of "Part:BBa K3380109"
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<partinfo>BBa_K3380109 short</partinfo> | <partinfo>BBa_K3380109 short</partinfo> | ||
| − | Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the | + | Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the native ''Cupriavidus Metallidurans'' CH34 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added. |
Taken from the work of Millacura, Valenzuela-Ortega and French 2019. | Taken from the work of Millacura, Valenzuela-Ortega and French 2019. | ||
Revision as of 22:52, 27 October 2020
C. Metallidurans promoter with Zn transcription factor binding site
Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the native Cupriavidus Metallidurans CH34 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
Taken from the work of Millacura, Valenzuela-Ortega and French 2019.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]