Difference between revisions of "Part:BBa K3606915"
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Sequence and Features
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Revision as of 22:51, 27 October 2020


P2 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P2 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5276
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5233
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5276
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5276
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
    Illegal NgoMIV site found at 1959
    Illegal AgeI site found at 2131
  • 1000
    COMPATIBLE WITH RFC[1000]