Difference between revisions of "Part:BBa K3380109"

 
Line 3: Line 3:
 
<partinfo>BBa_K3380109 short</partinfo>
 
<partinfo>BBa_K3380109 short</partinfo>
  
−
-
+
Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
 +
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
It can be used in a cell-free biosensor.
  
 
<!-- -->
 
<!-- -->

Revision as of 22:48, 27 October 2020


C. Metallidurans promoter with Zn transcription factor binding site

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.


Usage and Biology

It can be used in a cell-free biosensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]