Difference between revisions of "Part:BBa K165090"
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− | - | + | This is an example of the "R" repressor element in our genetic limiter circuit. It contains the Gli1 and LexA binding sites upstream of mCYC, regulating the expression of the LexA repressor. This is the element of the limiter that represses the gene of interest in the superthreshold state, being induced by G's inducer, the LexA activator in this case. It is repressed by the tau transcription factor when the system is subthreshold, in this case,the Gli1 repressor under control of the threshold-setting promoter. |
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Revision as of 04:55, 30 October 2008
Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306
This is an example of the "R" repressor element in our genetic limiter circuit. It contains the Gli1 and LexA binding sites upstream of mCYC, regulating the expression of the LexA repressor. This is the element of the limiter that represses the gene of interest in the superthreshold state, being induced by G's inducer, the LexA activator in this case. It is repressed by the tau transcription factor when the system is subthreshold, in this case,the Gli1 repressor under control of the threshold-setting promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 534
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 534
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2587
Illegal XhoI site found at 1
Illegal XhoI site found at 122
Illegal XhoI site found at 284 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 534
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 534
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 119
Illegal SapI.rc site found at 1758
Illegal SapI.rc site found at 2351