Difference between revisions of "Part:BBa K165090"

Revision as of 04:55, 30 October 2008

Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306

This is an example of the "R" repressor element in our genetic limiter circuit. It contains the Gli1 and LexA binding sites upstream of mCYC, regulating the expression of the LexA repressor. This is the element of the limiter that represses the gene of interest in the superthreshold state, being induced by G's inducer, the LexA activator in this case. It is repressed by the tau transcription factor when the system is subthreshold, in this case,the Gli1 repressor under control of the threshold-setting promoter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 534
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 534
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2587
Illegal XhoI site found at 1
Illegal XhoI site found at 122
Illegal XhoI site found at 284
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 534
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 534
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 119
Illegal SapI.rc site found at 1758
Illegal SapI.rc site found at 2351

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 __NOTOC__
 <partinfo>BBa_K165090 short</partinfo>
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+This is an example of the "R" repressor element in our genetic limiter circuit.  It contains the Gli1 and LexA binding sites upstream of mCYC, regulating the expression of the LexA repressor.  This is the element of the limiter that represses the gene of interest in the superthreshold state, being induced by G's inducer, the LexA activator in this case.  It is repressed by the tau transcription factor when the system is subthreshold, in this case,the Gli1 repressor under control of the threshold-setting promoter.
 <!-- Add more about the biology of this part here