Difference between revisions of "Part:BBa K3332078"
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Glyphosate can be metabolized into formaldehyde in ''E.coli''. Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter(<partinfo>BBa_Q04510</partinfo>) plays the role of gene inversion which reverses expression pattern when existing. MazF(<partinfo>BBa_K1720002</partinfo>) was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pR promoter inside inverter and cause cell death by protein toxicity. | Glyphosate can be metabolized into formaldehyde in ''E.coli''. Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter(<partinfo>BBa_Q04510</partinfo>) plays the role of gene inversion which reverses expression pattern when existing. MazF(<partinfo>BBa_K1720002</partinfo>) was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pR promoter inside inverter and cause cell death by protein toxicity. | ||
| − | <html>Glyphosate is metabolized into formaldehyde in the body of ''E.coli | + | <html>Glyphosate is metabolized into formaldehyde in the body of ''E.coli'' |
<figure> | <figure> | ||
<img src="https://2020.igem.org/wiki/images/9/9b/T--XMU-China--XMU-China_2020-pHCHO_inverter_mazF_B0015.png" width="60%" style="float:center"> | <img src="https://2020.igem.org/wiki/images/9/9b/T--XMU-China--XMU-China_2020-pHCHO_inverter_mazF_B0015.png" width="60%" style="float:center"> | ||
Revision as of 22:46, 27 October 2020
Formaldehyde promoter-Inverter-MazF-terminator
It can express toxin protein if there is no formaldehyde.
Usage and Biology
Glyphosate can be metabolized into formaldehyde in E.coli. Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter(BBa_Q04510) plays the role of gene inversion which reverses expression pattern when existing. MazF(BBa_K1720002) was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pR promoter inside inverter and cause cell death by protein toxicity.
Glyphosate is metabolized into formaldehyde in the body of ''E.coli''
Fig 1. pHCHO_inverter_mazF_B0015
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]