Difference between revisions of "Part:BBa I12007"
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Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI) | Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI) | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 22:33, 27 October 2020
Modified lambda Prm promoter (OR-3 obliterated)
Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
Usage and Biology
FIGURE 4. The relative fluorescence of WT (E.coli-wildtype), J23110 (E.coli-pSB1C3-K608010), HrtO (E.coli-pUC-GW-HrtO-GFP) and I12007 (E.coli-pSB1C3-I12007-GFP-pSB1K3-HrtO-cI).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium as blank. Relative fluorescence is shown in logarithmic axis, 2 to the power of n.
FIGURE 5. Calculating formula.
As shown in the figure, the expression strength of I12007 is about 8% of HrtO, under the activation of cI (promoter: HrtO), showing 92% of loss during signal transformation from HBS to BAS.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]