Difference between revisions of "Part:BBa K3629011"
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===Usage and Biology===
 
===Usage and Biology===
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This is the coding sequence for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including <i>Yarrowia lipolytica </i> -the chassis for our project. Particularly for <i> Y. lipolytica</i> there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this antibiotic 50-200µg/mL is added to media depending on the organism.
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The part is a key part of [https://2020.igem.org/Team:Calgary/Part_Collection our collection] and functions as a destination vector for other <i> Y. lipolytica</i> parts when cloned into a plasmid with a promoter and terminator sequence attached.
  
 
===Design===
 
===Design===

Revision as of 22:16, 27 October 2020


Nourseothricin resistance gene

Nourseothricin acetyltransferase (nat1) gene from Streptomyces noursei.

Usage and Biology

This is the coding sequence for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including Yarrowia lipolytica -the chassis for our project. Particularly for Y. lipolytica there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this antibiotic 50-200µg/mL is added to media depending on the organism.

The part is a key part of our collection and functions as a destination vector for other Y. lipolytica parts when cloned into a plasmid with a promoter and terminator sequence attached.

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References