Difference between revisions of "Part:BBa K3482030"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K3482030 short</partinfo>
 
<partinfo>BBa_K3482030 short</partinfo>
  
−
temp
+
azurin fused with pelB-5D secretion tag under the control of a pTet01 inucible promoter for protein expression.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
 +
 +
This part was assembled in a sponge plasmid working with a repressilator. The aim was to produce azurin in an oscillatory manner.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 22:02, 27 October 2020


Expression azurin with secretion tag under inducible pTetO1

azurin fused with pelB-5D secretion tag under the control of a pTet01 inucible promoter for protein expression.

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

This part was assembled in a sponge plasmid working with a repressilator. The aim was to produce azurin in an oscillatory manner.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 55
    Illegal NgoMIV site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]