Difference between revisions of "Part:BBa K3454046"

 
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<partinfo>BBa_K3454046 short</partinfo>
 
<partinfo>BBa_K3454046 short</partinfo>
  
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This is the coding sequence of AsCas12a with 6x His and MBP tags.
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This is the coding sequence of AsCas12a with 6x His and MBP tags.This part is the template for AsCas12a extraction and purfication for reaction system.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:43, 27 October 2020


AsCas12a_6xHis_MBP

This is the coding sequence of AsCas12a with 6x His and MBP tags.This part is the template for AsCas12a extraction and purfication for reaction system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 375
    Illegal BglII site found at 1571
    Illegal BglII site found at 3113
    Illegal BglII site found at 3422
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
    Illegal NgoMIV site found at 4657
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 73