Difference between revisions of "Part:BBa K1828999"

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<partinfo>BBa_K1828999 parameters</partinfo>
 
<partinfo>BBa_K1828999 parameters</partinfo>
 
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===HZAU-China 2020's contribution===
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<p><b>(The following experimental data are from the literature, not the actual data made by the team)</b></p>
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<p><b>We knew from the literature</b> that ComDE two component system exists in gram-positive bacteria plays an important role in quorum sensing in <i>Streptococcus mutans</i>. After being effected by CSP (competence stimulate peptide) secreted by <i>S.mutans</i>, the cascade reaction will take place in ComDE two component system, phosphorylated ComE then act on the conservative sequence which is called DR sequence (direct repeat sequence) (<b>Figure 1</b>) [1],the process changes the conformation of the region of these specific promoters, and makes it easy for RNA polymerase to combine to the specific region, thus induce the expression of genes coding mutacin like <i>comAB</i> and <i>comX </i>[2].</p>

Revision as of 21:19, 27 October 2020


nlmAB promoter

Description

The natural promoter found in Streptococcus mutans is regulated by the CSP concentration of the ability to stimulate the peptide. When no exogenous CSP is added, the expression strength is weak, and the addition of exogenous CSP can up-regulate the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



HZAU-China 2020's contribution

html> <body>

(The following experimental data are from the literature, not the actual data made by the team)

We knew from the literature that ComDE two component system exists in gram-positive bacteria plays an important role in quorum sensing in Streptococcus mutans. After being effected by CSP (competence stimulate peptide) secreted by S.mutans, the cascade reaction will take place in ComDE two component system, phosphorylated ComE then act on the conservative sequence which is called DR sequence (direct repeat sequence) (Figure 1) [1],the process changes the conformation of the region of these specific promoters, and makes it easy for RNA polymerase to combine to the specific region, thus induce the expression of genes coding mutacin like comAB and comX [2].