Difference between revisions of "Part:BBa K3657014:Design"
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===Design Notes=== | ===Design Notes=== | ||
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− | The aminoacid sequence was obtained from the Uniprot database ( | + | The aminoacid sequence was obtained from the Uniprot database (P03045). |
The nucletide sequence was then generated and codon optimised for <i>E. coli</i>. | The nucletide sequence was then generated and codon optimised for <i>E. coli</i>. | ||
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Revision as of 21:12, 27 October 2020
Antitermination protein N from Lambda Phage
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The aminoacid sequence was obtained from the Uniprot database (P03045). The nucletide sequence was then generated and codon optimised for E. coli.
Source
Bacteriophage lambda
References
Parks AR, Court C, Lubkowska L, Jin DJ, Kashlev M, Court DL. Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase. Nucleic Acids Res. 2014 May;42(9):5823-9. doi: 10.1093/nar/gku203. Epub 2014 Apr 7. PMID: 24711367; PMCID: PMC4027172.