Difference between revisions of "Part:BBa K3396018"

Revision as of 21:05, 27 October 2020

CMV->Kozak->GFPnano->FKBP->FKBP->P2A ->Truncated TRIM21->FRB->BGH-pA

This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP. And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 721

@@ Line 8: / Line 8: @@
 <!-- Add more about the biology of this part here
+This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP.
+And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.
 ===Usage and Biology===
+Our composite part BBa_K3396018 demonstrates how the Predator Pro system works. It regulates the target protein (EGFP) degradation under the control of external rapamycin signal.
 <!-- -->