Difference between revisions of "Part:BBa K3505018"

Revision as of 20:46, 27 October 2020

sfGFP GB compatible with B3-B5

Level 0 sfGFP

Fig.1:sfGFP

Fig.2:sfGFP uder control of pTRC

Usage and Biology

A reporter gene fluorescent protein

• Excitation at 485 nm
• Emission at 510 nm

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo.The sequence is cloned in pUPD2 BBa_K3505007and has overhangs compatible for GoldenBraid cloning. The CDS has position B3-B5.

Fig.3:The overhangs of this part in the Golden Braid Grammar

Verification of cloning

Fig.4:U4 C4 Level0 sfGFP Digested with EcoRI, PstI Expected bands 2029, 794

Experimental Use and Experience

This part is used in BBa_K3505030

Sequence and Features