Difference between revisions of "Part:BBa K3396013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsmBI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly. | This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsmBI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly. | ||
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| + | <html> | ||
| + | <p><img src="https://static.igem.org/mediawiki/parts/c/c1/T--NUDT_CHINA--Repla-1.png" alt="" width="500" /></p> | ||
| + | </html> | ||
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| + | Figure 1. Schematic representation of the Replaceable-1. | ||
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| + | ===Sequence and Features=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 20:27, 27 October 2020
Repleceable-1
This is the XTEN Linker gene flanked with specific restriction sites that could be recognized by BsmBI.
Usage and Biology
This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two BsmBI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.
Figure 1. Schematic representation of the Replaceable-1.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]