Difference between revisions of "Part:BBa K121012"

(→‎Usage and Biology)
(→‎Usage and Biology)
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We identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109.
 
We identified hill coefficient of Plac_GFP in BBa_I739003 in ''E. Coli'' strain JM109.
  
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[[Image:Transfer_Function_K121012.png|thumb|500px|right]]
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[[Image:Transfer_Function_K121012.png|thumb|500px|center|figure. Transfer Function of BBa_K121012. The data points represent the mean of experimental measurements. The solid green curve was calculated by fitting a Hill function to the experimental measurements.]]
  
 
The hill coefficient is 2.2.
 
The hill coefficient is 2.2.

Revision as of 03:16, 30 October 2008

Plac GFP on pSB6

pressure and IPTG inducible Plac (lacI regulated) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954

Usage and Biology

Identify Hill coefficient by IPTG induction

We identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.

figure. Transfer Function of BBa_K121012. The data points represent the mean of experimental measurements. The solid green curve was calculated by fitting a Hill function to the experimental measurements.

The hill coefficient is 2.2.