Difference between revisions of "Part:BBa K3424008:Design"
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
If you want to design another gene after using this terminator, make sure there are about 19 nucleotides in between the terminator and the new gene.
+
If you want to design another gene after using this terminator, make sure there are about 4 nucleotides in between the terminator and the next bio-part
  
 
===Source===
 
===Source===

Latest revision as of 19:49, 27 October 2020


Terminator_L3S2P21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to design another gene after using this terminator, make sure there are about 4 nucleotides in between the terminator and the next bio-part

Source

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE


References

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3