Difference between revisions of "Part:BBa K3365009:Design"
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===Source=== | ===Source=== | ||
| − | + | The lure3 sequence is predicted by our off-target predictor software built in 2019 and ligated to the promoter of the L-arabinose operon of E. coli (pBAD) by PCR. | |
===References=== | ===References=== | ||
Revision as of 19:23, 27 October 2020
Lure3 sequence downstream of pBAD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 74
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
Design Notes
The lure3 sequence is contained in the reverse primer.
Source
The lure3 sequence is predicted by our off-target predictor software built in 2019 and ligated to the promoter of the L-arabinose operon of E. coli (pBAD) by PCR.